摘要：背景:本研究的目的是确定抗磷脂酰丝氨酸/凝血酶原抗体(APS/PT)与中国抗磷脂综合征(APS)和血清阴性APS(SNAPS)患者血栓形成和妊娠丢失的患病率和临床相关性。方法:对186例中国APS(原发性67例，继发性119例)，48例SNAPS，176例疾病对照者(系统性红斑狼疮79例，干燥综合症29例，强直性脊柱炎30例，类风湿关节炎38例)和90例健康献血者进行检查。用酶联免疫吸附试验检测lgG和IgM aPS/PT，IgG/IgM/IgA aCL以及IgG/IgM/IgA anti-β2GPI。结果:160例APS患者(86.0%)至少有一个aPS/PT阳性。135例(72.6%)IgGaPS/PT阳性，124/186例(66.7%) IgM aPS/PT阳性，99例(53.2%)两种抗体均阳性。大约一半的SNAPS患者的IgG和/或IgM aPS/PT呈阳性。IgGaPS/PT与静脉血栓形成事件(OR=6.72)和IgG/IgM aPS/PT与妊娠丢失(OR=9.44)有显著的相关性。伴血栓形成和伴胎儿丢失的APS患者之间IgM aPS/PT水平有显著差异(p = 0.014)。IgG/IgMaPS/PT与狼疮抗凝物的相关性非常显著(p<0.001)。当两者均为阳性时，APS的OR为101.6。值得注意的是，91.95%(80/87)的LAC阳性标本的IgG和/或IgM aPS/PT呈阳性，表明当LAC检测不可用时aPS/PT是一种有效的选择。结论: aPS/PT抗体检测对中国APS患者具有较高的诊断价值，可检出一些标准标志物阴性的APS患者，可作为静脉血栓形成和产科并发症的潜在风险预测指标。

附原文：

Background:The aim of the study was to determine the prevalence and clinical associationsof antiphosphatidyl- serine/prothrombin antibodies (aPS/PT) with thrombosis andpregnancy loss in Chinese patients with antiphospho- lipid syndrome (APS) andseronegative APS (SNAPS). Methods: One hundred and eighty six Chinese patientswith APS (67 primary, 119 secondary), 48 with SNAPS, 176 disease controls (79systemic lupus erythematosus [SLE], 29 Sjogren’s syndrome [SS], 30 ankylosingspon- dylitis [AS], 38 rheumatoid arthritis [RA]) and 90 healthy donors wereexamined. IgG and IgM aPS/PT, IgG/IgM/IgA anticardiolipin (aCL) and IgG/IgM/IgAanti-β2-glycoproteinI (anti-β2GPI) antibodies were tested by ELISA. Results: One hundred and sixty(86.0%) of APS patients were positive for at least one aPS/PT isotype. Onehundred and thirty five (72.6%) were positive for IgG aPS/PT, 124/186 (66.7%)positive for IgM aPS/PT and 99 (53.2%) positive for both. Approximately half ofthe SNAPS patients were positive for IgG and/or IgM aPS/PT. Highly significantassociations between IgG aPS/PT and venous thrombotic events (odds ratio [OR] =6.72) and IgG/IgM aPS/PT and pregnancy loss (OR = 9.44) were found. Levels ofIgM aPS/ PT were significantly different in APS patients with throm- boticmanifestations and those with fetal loss (p = 0.014). The association betweenIgG/IgM aPS/PT and lupus anti- coagulant (LAC) was highly significant (p <0.001). When both were positive, the OR for APS was 101.6. Notably, 91.95%(80/87) of LAC-positive specimens were positive for IgG and/or IgM aPS/PT,suggesting aPS/PT is an effec- tive option when LAC testing is not available.Conclusions: Anti-PS/PT antibody assays demonstrated high diagnosticperformance for Chinese patients with APS, detected some APS patients negativefor criteria markers and may serve as potential risk predictors for venousthrombosis and obstetric complications. (Shi,Zheng et al. 2018).

引自：Shi, H., H. Zheng, Y.-F. Yin, Q.-Y.Hu, J.-L. Teng, Y. Sun, H.-L. Liu, X.-B. Cheng, J.-N. Ye, Y.-T. Su, X.-Y. Wu,J.-F. Zhou, G. L. Norman, H.-Y. Gong, X.-M. Shi, Y.-B. Peng, X.-F. Wang andC.-D. Yang (2018). "Antiphosphatidylserine/prothrombin antibodies (aPS/PT)as potential diagnostic markers and risk predictors of venous thrombosis andobstetric complications in antiphospholipid syndrome."ClinicalChemistry and Laboratory Medicine (CCLM)56(4): 614-624.