摘要：

复发性流产发生在1-5%的产妇中。复发性流产(RAS)的持续治疗和研究是非常昂贵的，这是医生和患者面临的非常严重的问题。本研究的目的是检测复发性流产患者和健康对照组血清中的蛋白质表达谱，并确定该疾病的潜在生物标志物。使用人L -507和L- 493结合的1000μm蛋白芯片。微阵列数据显示，与健康对照组相比，RSA患者血清中八种蛋白表达水平显著上调，143种蛋白下调。在更大的患者和对照样本队列中，ELISA单独验证了151个蛋白中的5个，证明胰岛素样生长因子结合蛋白相关蛋白1(IFGBP-rp1)/ IGFBP-7，Dickkopf相关蛋白3 (Dkk3)，RSA患者中晚期糖基化终产物(RAGE)和血管生成素-2水平的受体显著增加。通过受试者操作特征曲线来分析和计算敏感性和特异性性，结果显示，敏感性分别是0.881, 0.823, 0.79 and 0.814诊断特异性的切点分别是胰岛素样生长因子结合蛋白相关蛋白195.44 ng/ml，Dickkopf相关蛋白3 为32.84 ng/ml，RSA患者中晚期糖基化终产物为147.27 ng/ml，血管生成素-2水平的受体为441.40 ng/ml。目前的研究表明，这四种蛋白质在RSA样本中下调，可能可作为预测和诊断RSA的生物标志物。为了进一步验证这些标记的诊断价值，需要大规模队列的后续研究。

附原文：

AbstractRecurrent spontaneous abortion (RSA) occurs in 1?6?25% of parturients. The sustained therapy and research for RSA is expensive, which is a serious issue faced by both patients and doctors. The aim of the present study was to detect protein expression profiles in the serum of RSA patients and healthy controls, and to identify potential biomarkers for this disease. A 1,000?6?2protein microarray consisting of a combination of Human L?6?2507 and L?6?2493 was used. The microarray data revealed that eight serum protein expression levels were significantly upregulated and 143 proteins were downregulated in RSA patients compared with the healthy controls. ELISA individually validated 5 of these 151 proteins in a larger cohort of patients and control samples, demonstrating a significant decrease in insulin?6?2like growth factor?6?2binding protein?6?2related protein 1 (IFGBP?6?2rp1)/IGFBP?6?27, Dickkopf?6?2related protein 3 (Dkk3), receptor for advanced glycation end products (RAGE) and angiopoietin?6?22 levels in patients with RSA. Sensitivity and specificity analyses were calculated by a receiver operating characteristics curve, and were revealed to be 0.881, 0.823, 0.79 and 0.814, with diagnostic cut?6?2off points of 95.44 ng/ml for IFGBP?6?2rp1, 32.84 ng/ml for Dkk3, 147.27 ng/ml for RAGE and 441.40 ng/ml for angiopoietin?6?22. The present study indicated that these four proteins were downregulated in RSA samples and may be useful as biomarkers for the prediction and diagnosis of RSA. Subsequent studies in larger?6?2scale cohorts are required to further validate the diagnostic value of these markers.

引自：Wu Y et al.Serum biomarker analysis in patients with recurrent spontaneous abortion.Mol Med Rep. 2017 Sep;16(3):2367-2378. doi: 10.3892/mmr.2017.6890. Epub 2017 Jun 30.